⚠️ Research Use Only. Not validated for clinical diagnostic use.

VigyanLLM's multiple sequence alignment tool uses Clustal Omega for accurate alignment of DNA, RNA, or protein sequences. The MSA engine outputs a consensus sequence, phylogenetic tree, and position-specific scoring matrices for downstream analysis.

InputOutput
FASTA sequencesAligned sequences with gap placement
Clustal/Nexus formatConsensus sequence & conservation scores
Distance matrixPhylogenetic tree (Newick format)
Guide treePSSM for motif discovery

Sequences

Paste Upload
0 sequences · 0 total bp

Alignment

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Start by pasting sequences or uploading a FASTA file (up to 100,000 sequences)

Frequently Asked Questions About Multiple Sequence Alignment (MSA) Tool

Everything you need to know about using this tool

Is the MSA tool free to use?

The MSA tool offers 5 free analyses per user. After that, a subscription is required. Each alignment uses backend compute resources for accurate ClustalW/MUSCLE-based alignment.

What is multiple sequence alignment?

MSA aligns three or more biological sequences to identify conserved regions, mutations, and evolutionary relationships. It is essential for phylogenetic analysis, conserved domain discovery, and variant analysis.

How many sequences can I align at once?

You can align up to 50 sequences at a time. Longer sequences and larger datasets produce more comprehensive results.

Do I need to create an account?

No, you can use the MSA tool without an account for your first 5 analyses. Creating an account allows you to track usage and manage subscriptions.

What alignment methods are supported?

The tool supports ClustalW and MUSCLE alignment algorithms with configurable gap penalties and scoring matrices.

How do I subscribe for unlimited access?

You can subscribe through the VPrime 2.0 platform. Subscriptions start at ₹99/day for the Daily Pass with monthly plans from ₹2,499/month.