Sovereign Biomedical AI

Sovereign Biomedical AI
on your infrastructure

VigyanLLM is an Indian sovereign Biotechnology artificial intelligence platform developed for on-premises biomedical research. It provides autonomous primer design, CRISPR guide RNA analysis, and GPU-accelerated molecular docking via Docker (Linux) deployment, with free academic access for Indian institutions and global research labs, ensuring data sovereignty, on-premises artificial intelligence, air-gapped genomics, DPDP-compliant architecture, US data center deployment.

🌐 Data centers in India & North America 🔒 Air-gapped network support 🛡️ DPDP-compliant architecture 📦 Deploy via Docker anywhere
Designs Run
Researchers
Validated Primers
Partner Orgs
PyTorch Docker Native CUDA Accelerated FASTA/GenBank Compatible REST API

Integrated molecular biology suite

Production-ready modules covering primer design, sequence analysis, genome engineering, and structural biology — all on-premise.

Primer & PCR Design

24-step primer design pipeline with thermodynamic validation, BLAST specificity, SNP screening, and multiplex optimization.

  • Primer & Probe Design
  • PCR Analysis & Multiplex Optimization
  • Tm & GC Calculators

Sequence Analysis

Nucleotide BLAST, multiple sequence alignment, and sequence search across public and custom reference databases.

  • Sequence Search by Name / Accession
  • BLAST with Configurable Databases
  • MSA with Conservation Scoring

Genome Engineering

CRISPR guide RNA design with off-target scoring, specificity validation, and exon-level targeting across genomes.

  • Guide RNA Design & Scoring
  • Off-Target Specificity Check
  • Exon / Gene Targeting

Structural Biology

GPU-accelerated molecular docking with scoring functions, binding site analysis, and ligand optimization.

  • Protein-Ligand Docking
  • Scoring & Binding Analysis
  • GPU-Accelerated Computation

Production-ready modules

Every tool is designed for bench scientists — no cloud dependency, no data leaving your institution.

VigyanLLM Primer
24-step primer and probe design pipeline — thermodynamics, BLAST, SNP filter, repeat masking, multiplex check.
Open tool →
Sequence Search
Search by name, accession, or paste raw DNA. Browse with cross-reference links.
Open tool →
BLAST
Nucleotide BLAST with configurable databases, organism filters, and alignment scoring.
Open tool →
MSA
Multiple sequence alignment with color-coded conservation, gap analysis, FASTA/Clustal export.
Open tool →
PCR Analysis
Multiplex optimization, TaqMan probe design, thermocycling profile generation.
Open tool →
CRISPR Analysis
Guide RNA design, off-target scoring, specificity validation, exon targeting.
Open tool →Coming Soon
Tm Calculator
Nearest-neighbor melting temperature with salt correction and thermodynamics.
Open tool →
GC
GC Calculator
GC content, melting temperature, and molecular weight for DNA/RNA sequences.
Open tool →
Protein Docking
GPU-accelerated docking, scoring functions, and binding site analysis.
Open tool →

Partnership programs

Designed for academic institutions and enterprise labs that need sovereign AI infrastructure.

Academic Partnership

Free 30-day full-platform access for qualifying university labs. No feature gates, no throttling, full export.

Learn more →

Enterprise Inquiry

On-premise deployment, dedicated support, custom integrations, and SLA-backed infrastructure for your lab.

Contact us →

Verified Partner

Technology partnerships, reseller programs, and integration collaborations for labs and enterprises worldwide.

Apply now →

Built for researchers, by researchers

Every tool is designed around a simple principle: sovereign AI infrastructure that puts labs in control of their data.

Infrastructure

100% on-premise, zero external API calls

All computation runs on your hardware. No data leaves your institution. No per-query costs, no internet dependency.

Validation

24-step biophysical validation pipeline

Every primer pair passes through thermodynamic checks, BLAST specificity, secondary structure, and repeat masking.

Export

Audit-ready reports, multiple formats

Export results as PDF, CSV, or JSON. IDT and Twist-compatible format for direct oligo ordering.

Sovereignty

Built in India. Sovereign AI infrastructure.

Designed and developed in India for global biomedical research. Full data sovereignty, no foreign cloud dependency.

Scale

From single primers to high-throughput panels

Whether designing one primer pair or a 96-well panel, the pipeline handles batch design with consistent validation.

Access

Direct academic partnership program

Free access for qualifying university labs. 30-day full-platform evaluation with no feature gates.

10,000+
Primer pairs designed
500+
Active researchers
99.5%
Pipeline uptime

"The 24-check validation caught specificity issues that our previous workflow missed. The PDF audit report is a game-changer for lab documentation."

— Lab Director, Academic Research Institute

Is VigyanLLM free? Trusted primer design, answered

Is VigyanLLM free to use?

Yes. Running any of the tools — primer design, BLAST, MSA, docking, Tm and GC calculators — is free for anonymous visitors, with no login required at the point of use. Free accounts get a daily allowance of analyses at no cost. Paid plans only add persistence and scale: saving results, PDF/PPT export, batch design, and higher daily limits. There is a free academic tier for qualifying university labs.

Can I trust a free primer design tool for research?

Reliability comes from the validation model, not the price tag. VigyanLLM uses the same shared chemistry as NCBI Primer-BLAST — SantaLucia 1998 nearest-neighbor thermodynamics for Tm, Primer3 v2.6.1 for hairpin and dimer screening, and BLAST-level specificity. Our public validation benchmark independently reproduces the reported Tm of three published primer sets, confirming agreement within tool-to-tool variation.

How do I access a reliable online primer designer in research?

You can start at vigyanllm.in/primer without signing up. Paste your template, choose PCR type and amplicon window, and the pipeline runs Design Automatically. For diagnostics and clinical use, results come with audit-ready reports and a validation record tied to published primers — evidence a reviewer can verify.

Is VigyanLLM restricted to India only?

No. VigyanLLM is designed and developed in India and is free for qualifying Indian academic institutions, but the platform is open globally to all researchers. Tools run in the browser with no US cloud dependency by default, so data stays where your lab wants it, wherever you are.

Research Topics

Primer Design
Thermodynamic models, specificity checks, probe design
Bioinformatics Tools
BLAST, MSA, sequence analysis, alignments
Molecular Docking
Protein-ligand interactions, scoring, preparation
PCR Amplification
Protocols, troubleshooting, optimization
Gene Expression
RT-qPCR, quantification, normalization
CRISPR & Genome Editing
gRNA design, off-target analysis, editing tools

See It in Action

Primer design tutorial — from sequence input to validated report in under 5 minutes

Latest from the Blog

View all posts →
PCR
PCR Steps: Complete Thermal Cycling Protocol
Denaturation, annealing, extension — step by step with real temperatures
Primer-BLAST
NCBI Primer-BLAST Guide (2026)
Step-by-step with examples for SARS-CoV-2 and human targets
qPCR
RT-PCR vs qPCR: What's the Difference?
Side-by-side comparison with decision guide for choosing the right method
Troubleshooting
Primer Dimer Fix: Lab Notebook
Real diagnostic data, root causes, and actionable fixes